Scientific Publications with referee

An immunological study of glycosaminoglycans in the connective tissue of bovine and cod skeletal muscle.

 

Vi har undersøkt ekstracellulær matriks sammensetningen i kjøtt og fiskefilet for å spore hvilke komponenter som er involvert i mørhet hos storfe og spalting hos torsk. Dette har vi gjort ved å sammenligne sammensetningen og oppbygningen av bindevevet hos de to artene. Vi fant betydelige forskjeller, særlig i keratansulfat, som kan være ansvarlig for forskjellene i tekstur. Ytterligere studier er på gang for å klargjøre dette.

Facts

Year 2006
Abstract The presence of sulfated glycosaminoglycans (GAGs) was demonstrated in the connective tissue of bovine and cod skeletal muscle by histochemical staining using Alcian blue added MgCl2 (0.06 M and 0.4 M, respectively). For further identification of the sulfated GAGs, a panel of monoclonal antibodies, 1B5, 2B6, 3B3 and 5D4 was used that recognizes epitopes in chondroitin-0-sulfate (C0S), chondroitin-4-sulfate/dermatan sulfate (C4S/DS), chondroitin-6-sulfate (C6S) and keratan sulfate (KS), respectively. Light microscopy and Western blotting techniques showed that in bovine and cod muscle C0S and C6S were primarily localized pericellularly, whereas cod exhibited a more intermittent staining. C4S was expressed around the separate cells and also in the perimysium and myocommata. In contrast to bovine muscle, which hardly expressed highly sulfated KS, cod exhibited a very strong and consistent staining. Western blotting showed that C0S and C6S were mainly associated with proteoglycans (PGs) of high molecular sizes in both species. Contrary to bovine muscle, C4S in cod was associated with molecules of various sizes. Both cod and bovine muscle contained KSPGs of similar sizes as C4S. KSPGs of different sizes and buoyant densities, sensitive to keratanase I and II were found expressed in cod.
Reference Hannesson, K.O., Tingbø, M.G., Olsen, R.L., Enersen, G., Bævre, A.B., Ofstad, R. 2007. An immunological study of glycosaminoglycans in the connective tissue of bovine and cod skeletal muscle. Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, Vol 146, pp 512-520.
Publisher Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology,

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