Scientific Publications with referee

Identification and distribution of heparan sulfate proteoglycans in the white muscle of Atlantic cod (Gadus morhua L.) and spotted wolffish (Anarhichas minor O.)

 

Identifisering og distribusjon av heparansulfat proteoglykaner i hvit muskel hos torsk og steinbit

Heparansulfat proteoglykanene perlekan og agrin ble identifisert i hvit muskel hos to arter kaldtvannsfisk (torsk og steinbit). Proteoglykaner degraderes i muskulært bindevev etter slakt, og heparansulfat proteoglykanene perlekan og agrin er viktige matriksmolekyler.

Immunologiske teknikker som dot blot og western blot ble brukt, i tillegg til innmerking med radioaktivt sulfat. 35S-sulfat ble injisert i fisken slik at sulfatet ble inkorporert i karbohydratsidekjedene til proteoglykanene. HNO2 behandling kuttet HS sidekjedene, og reduksjon i radioaktivitet ble målt etter DEAE kromatografi vha scintillasjonstelling. Distribusjonen i vev ble demonstrert vha immunhistokjemiske teknikker. Perlekan viste svært ulik distribusjon i vevet til fisk kontra det man har sett før hos pattedyr.

Tingbø, M.G., Kolset, S.O., Ofstad, R., Enersen, G., Hannesson, K. 2006. Identification and distribution of heparan sulfate proteoglycans in the white muscle of Atlantic cod (Gadus morhua L.) and spotted wolffish (Anarhichas minor O.) Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, Vol 143, 4, pp 441-452.

Facts

Year 2005
Abstract Heparan sulfate proteoglycans (HSPGs) were identified in pre rigor muscle of two species of cold water fish, Atlantic cod (Gadus morhua) and spotted wolffish (Anarhichas minor) by biochemical and immunological methods. The distribution was described by immunohistology. Special emphasize was directed to the extracellular matrix (ECM) HSPGs perlecan and agrin. In vivo 35S-sulfate labeling combined with ultracentrifugation in CsCl2, DEAE chromatography and scintillation counting of the eluates, revealed that the content of 35S labeled PGs was much higher in wolffish than in cod. A considerable proportion of the 35S-sulfated PGs in both species was HSPG, as judged by nitrous acid degradation. HSPG represented, however, a higher proportion of the 35S-sulfated PGs in cod compared to wolffish. Dot blot and electrophoresis/western blot using two different HS- mAbs, 10E4 and HepSS-1 indicated structural differences in the HS-chains of the PGs present. This observation was strengthened by immunohistochemistry, showing that both mAbs detected epitopes in the pericellular area, but the staining patterns were not superimposable. Two different agrin isoforms were identified in both species. Furthermore, in the white muscle of both cod and wolffish, perlecan mAb (A7L6) showed positive staining restricted to the transition between myocommata and myofibers.
Reference Tingbø, M.G., Kolset, S.O., Ofstad, R., Enersen, G., Hannesson, K. 2006. Identification and distribution of heparan sulfate proteoglycans in the white muscle of Atlantic cod (Gadus morhua L.) and spotted wolffish (Anarhichas minor O.) Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, Vol 143, 4, pp 441-452.
Publisher Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology,

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