Scientific Publications with referee

16S rDNA analyses of the cyanobacterial microbiota through the water-column in a boreal lake with a metalimnic planktothrix population.

 

Blågrønnalger kan være et stort problem med hensyn på bruk av vann i industri eller landbruk. Steinsfjorden har en spesiell forekomst av giftproduserenede alger. Vi har undersøkt disse algene ved bruk av moderne DNA matrise teknologi i kombinasjon av måling av klorofyll fra algene. Det nye med våre analyser er at vi har analysert bakteriene i et transekt gjennom vannkolonen. Dette studiet har gitt en bedre forståelse av giftalgene som igjen kan brukes til å bekjempe masseforekomster.

Facts

Year 2005
Abstract The Planktothrix population in Lake Steinsfjord has attracted particular attention due to the potential development of toxic blooms. This population is special in the sense that mass developments of Planktothrix occur in the metalimnion. We investigated the distribution of Planktothrix, as well as other cyanobacteria, through the water-column during a Planktothrix mass development at 10-16 m depth. The analyses were done by chlorophyll measurements, microscopy and by a recently developed 16S rDNA array-based method. These analyses showed that Planktothrix dominated the cyanobacterial community at 11 m, while cyanobacteria belonging to the order Nostocales were predominant at 4 m. The combination of analytic methods presented in this work provides a powerful tool to analyze cyanobacterial communities. We have developed a concept that enables both relative (16S rDNA array analyses) and absolute quantification (chlorophyll a measurements) of cyanobacteria through water-columns. Such approaches will be important in better understanding cyanobacterial microbiota and bloom dynamics.
Reference Rudi, K., Skulberg, O.M., Jakobsen, K.S. 2005. 16S rDNA analyses of the cyanobacterial microbiota through the water-column in a boreal lake with a metalimnic planktothrix population. Preparative Biochemistry & Biotechnology, Vol 35, pp 301-312.
Publisher Preparative Biochemistry & Biotechnology,