Vitenskapelige artikler m/referee

Multivariate image analysis of a set of FTIR micro-spectroscopy images of aged bovine muscle tissue combining image and design information.

 

I denne publikasjonen brukte vi FTIR mikroskopi for å studere mørning av biff. Forskjellige dyr ble fulgt over en tidsperiode, og det ble vist at de største forandringene i bindevev skjedde i de første to dagene. Nye algoritmer for analyse av FTIR bilder ble presentert.

Nøkkeldata

Årstall 2007
Abstract In this paper we present an algorithm for analysing sets of FTIR microscopic images of tissue sections. The proposed approach allows investigating sets of many FTIR tissue images both with respect to sample information (variation from image to image) and spatial variations of tissues (variation within the image). The algorithm is applied to FTIR microscopy images of beef loin muscles containing myofiber and connective tissue regions. The FTIR microscopy images are taken of sub-samples from 5 different beef loin muscles that were aged for 4 different lengths of times. The images were investigated regarding variation due to the ageing length and due the homogeneity of the connective tissue regions. The presented algorithm consists of the following main elements: (1) Pre-processing of the spectra to overcome large quality differences in FTIR spectra and differences due to scatter effects. (2) Identification of connective tissue regions in every image. (3) Labelling of every connective tissue spectrum with respect to its location in the connective tissue region. (4) Analysis of variations in the FTIR microscopic images in regard to ageing time and pixel position of the spectra in the connective tissue region. Important spectral parameters characterising collagen and proteoglycan structure were determined.
Referanse Kohler, A., Bertrand, D., Martens, H., Hannesson, K.O., Kirschner, C., Ofstad, R. 2007. Multivariate image analysis of a set of FTIR micro-spectroscopy images of aged bovine muscle tissue combining image and design information. Analytical and Bioanalytical Chemistry, Vol 389, pp 1143-1153.
Utgiver Analytical and Bioanalytical Chemistry,

Relaterte personer

  • Achim Kohler

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  • Harald Martens

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  • Ragni Ofstad

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