Vitenskapelige artikler m/referee

Estimating and correcting Mie scattering in synchrotron-based microscopic FTIR spectra by extended multiplicative signal correction (EMSC).

Nøkkeldata

Årstall 2007
Abstract We present an approach for estimating and correcting Mie scattering occurring in infrared spectra of single cells, at diffraction limited probe size, as in synchrotron based microscopic. The Mie scattering is modeled by the formulae developed by (Van De Hulst 1957) and estimated before being separated out from the vibrational absorption using extended multiplicative signal correction (EMSC). Since the Mie scattering is depending non-linearly on the product of radius and refractive index of the medium/sphere causing it, a new method based on principal component analysis (PCA) was developed for estimating the Mie scattering by EMSC for unknown radius and refractive index of the Mie scatterer. The product of radius and refractive index is subsequently estimated. It was demonstrated that nuclei and cells are the most possible candidates for Mie scattering.
Referanse Kohler, A., Sulé-Suso, J., Sockalingum, G.D., Tobin, M., Bahrami, F., Yang, Y., Pijanka, J., Dumas, P., Cotte, M., van Pittius, D.G., Parkes, G., Martens, H. 2008. Estimating and correcting Mie scattering in synchrotron-based microscopic FTIR spectra by extended multiplicative signal correction (EMSC). Applied Spectroscopy, Vol 62, Issue 3, pp 259-266.
Utgiver Applied Spectroscopy,

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  • Achim Kohler

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  • Harald Martens

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